Validation of GENE UP® EHEC Method for STEC Detection Using New Molecular Makers and Isolation on IDEA™ STEC Agar
By Astrid Cariou , Florian Quero , Cécile Bernez , Yohan Huitric , Tamara Roger-Caedoso , David Tomas ADRIA, Quimper, France; bioMérieux.
Poster presented at VTEC 2026 in Aberdeen, Scotland.
Introduction and Objectives
EHEC are major foodborne pathogens requiring rapid and accurate detection Novel Molecular markers (espK / espV) are suggested to improve the specificity of EHEC by detecting colocalization of stx and eae genes in the same cell [1] [2].
IDEA™ STEC Agar is designed to enable faster, clearer, and more reliable isolation of STEC and particularly EHEC strains based on detection of Shiga toxin production. New workflow combines GENE-UP® Pathogenic E. coli kit (PEC) + IDEA™ STEC Agar in the previous validated method including stx and eae and TOP 7 serogroup detection.
The objective of the study is to validate new workflow vs ISO 131136: 2012 following ISO 16140-02: 2016 E. coli kit (PEC) + IDEA™ STEC Agar.
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