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Scientific Posters

See bioMérieux’s scientific posters showcasing research and rapid detection methods to advance food safety, quality control, and traceability.

BIOBALL

Which QC strains to use for minimizing risk of false positives due to cross-contamination in a lab?

The use of traceable culture collection strains, including pathogenic ones, is necessary in food as well as water and environmental laboratories for various applications, including: • Daily or regular Quality Control (QC) of methods, mandatory for accredited labs • Performance testing of culture media and reagents • Verification and in-house validation of methods (e.g., according to ISO 16140-3) However, the use of (pathogenic) strains, which often require culture and inoc ulum preparations, can lead to cross-contamination of routine samples.

New EHEC Markers

Standard molecular methods (without immunoconcentration step) of STEC detection in food matrices based on stx and eae genes co-detection and Top5/7 serogroup associated genes may lead to identify a significant number of false positives without detection of all enterohemorrhagic Escherichia coli (EHEC) potentially present. These major limits may cause either a substantial economic loss for food industry and a food safety risk for consumers.

Reduction Of Environmental Footprint And Time To Result For Listeria Monocytogenes Detection By Cultural Iso 16140-2 Validated Method

Detection of Listeria monocytogenes in food and environmental samples following ISO 11290-1:2017 includes sample preparation step using 1/10 dilution followed by secondary enrichment and isolation, requiring four days to release negative results...

Understanding Food Production Traceability

In order to meet the growing expectations of consumers and the vigilance of regulation authorities, the food industry now uses new technology to improve food traceability.

Rapid Detection of Spoilage Microorganisms in Fruit Juices

The objective is to validate one rapid detection method of spoilage microorganisms, including molds, yeasts, lactic acid and acetic acid bacteria in low pH fruit juice ingredients The bioMérieux method D COUNT ® 50 based on Flow Cytometry technology, allowed a significant reduction of time to results from 6 days to 3 days providing valid and equivalent results to the reference cultural method (International Fruit and Vegetable Juice Association IFU 2 C).

Validation Study Of A Rapid Method For The Detection Of Salmonella Spp In Raw Materials For Chocolate Industries Using GENE-UP® PCR and EGENE-UP® Easyprep Solution.

The chocolate industry is particularly concerned by Salmonella spp. contaminations in raw materials derived from cocoa beans. Salmonella is ubiquitous and present in the environment and can persist in cocoa products along the process of cocoa beans transformation. When present in cocoa products, Salmonella strains are subjected to a high level of stress and can be injured, making complex the detection by conventional and rapid methods. Moreover, the need of a rapid method in one shift is important due to specific manufacturing constraints. For this reason, bioMérieux has developed a new protocol to reduce significantly the timeto-result from 24 hours for current GENE-UP® SLM2 alternative method to 12 hours for 375g raw materials for chocolate industries.

Image Poster - How to implement genetically modified microorganisms for routine quality control of methods in labs?

Genetically modified microorganisms for routine methods quality control

According to ISO/IEC 17025, testing laboratories should ensure they operate competently and are able to generate valid results. Implementation of Quality Control or Verification of methods requires the use of strains. Genetically Modified Microorganisms (GMM) (e.g., GFP tagged organisms) offer a less risky approach minimizing the impact of cross contamination events securing laboratory results.

Performance Evaluation of GENE-UP NUTRAPLEX PRO on Nutraceuticals and Dietary Supplements

Rapid methods such as PCR have been making in-roads into the routine nutraceutical and dietary supplements testing, the potential of multiplex PCR for routine detection of multiple pathogens has yet to be ascertained. The diversity and ever-growing list of matrices in these industries further exacerbates the challenges for such methods and thereby the method compatibility should be reviewed with the adoption of new technology.

Performance Evaluation of GENE-UP^®

In the United States, the production and consumption of green leafy produce has been steadily rising. The most recent attribution estimates from Centers for Disease Control and Prevention (2013) indicated leafy produce to be responsible for the highest amount of foodborne illnesses and among the top five causes of foodborne hospitalizations and deaths. Between 1973 and 2012, Salmonella and Shiga toxinproducing Escherichia coli (STEC) species were the top bacterial pathogens responsible for outbreaks caused by leafy vegetables.

Evaluation of the GENE-UP® Salmonella (SLM) Assay

Infection with nontyphoidal Salmonella bacteria is a leading cause of foodborne deaths and hospitalizations in the United States each year. Salmonella outbreaks associated with ground beef have persisted and in fact several recent large outbreaks have heightened the ongoing concerns of its safety. The bioMérieux GENE-UP® Salmonella (SLM) Kit is a real-time PCR Assay capable of detecting low levels of Salmonella even in the presence of high background products such as ground beef.

The Fit for Purpose Evaluation of the BACT/ALERT® System

Learn more about microbial detection in a variety of plant-based alternative dairy beverages.

Performance Evaluation of Fluorescence Resonance Energy Transfer-Based (FRET)

The incidence of campylobacteriosis has been steadily rising in the US since 2011 (FoodNet), and despite the ongoing mitigation steps by the poultry industry, the testing raw poultry remains an effective strategy to surveil the incidence of Campylobacter sp.

Development of a Targeted Real-Time qPCR

Ensuring the quality of beverage products is complicated via traditional microbial detection methods due to a varied microbiome. Detailed analysis of spoilage organisms allows for a specified assay to rapidly and efficiently monitor for threats to product quality.

Validation of a Real-Time PCR Assay for Rapid Quantification of Salmonella

Rapid Salmonella quantification methods that are comparable to the classical most probable number (MPN) method would provide industry with the ability to make decisions about product disposition. The GENE-UP® QUANT Salmonella™ (QTS) assay utilizes an enrichment free sample prep paired with a multiplex detection method targeting a highly conserved pan-Salmonella genetic signature and an internal amplification control on the GENE-UP® providing Salmonella quantification in raw poultry in less than 4 h.

Use of New Markers for Precise Detection of Pathogenic Shiga Toxin-Producing Escherichia coli.

The USDA-FSIS has indicated that only ~10% of beef trim samples that contain stx, eae, and a serogroup gene can be culture confirmed. Newly commercialized virulence-associated biomarkers that are present in potentially pathogenic eae-positive STEC may reduce the number of non-culture confirmable PCR screening results and increase the reliability of STEC screening assays.

AOAC Emergency Response Validation of the TEMPO® YM Method

TEMPO® YM is an AOAC® Performance Tested Method℠ (PTM) approved for the automated enumeration of yeasts and molds in a variety of foods. The method utilizes a selective dehydrated culture medium and a Yeast and Mold (YM) enumeration card containing 48 wells across three dilutions for the automatic determination of the Most Probable Number (MPN).